久久久久久亚洲精品,SM调教室虐男受调教H打开腿,欧洲-级毛片内射八十老太婆,护士趴下光屁股翘臀被打的作文

您好,歡迎進入上海力敏實業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁 >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 常規(guī)生化試劑 >> sigma現(xiàn)貨代理Dispase II D-4693

sigma現(xiàn)貨代理Dispase II D-4693

  • 更新時間:  2023-07-26
  • 產(chǎn)品型號:  
  • 簡單描述
  • sigma現(xiàn)貨代理Dispase II D-4693
    Related CategoriesApplication Index, Biochemicals and Reagents, Cell Biology, Dissociation, Enzymes, Inhibitors, and Substrates,
    More...
    activity ≥0.5 units/mg solid
    sol
詳細(xì)介紹

sigma現(xiàn)貨代理Dispase II D-4693

Description

Analysis Note

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.

Preparation Note

A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate and milk sugar

Application

The enzyme from Sigma has been used in developing a protocol for ex vivoculture of mouse embryonic mammary buds.[5] It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart.[6] It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.[1]
Dispase II has been used for Fluorescence-Activated Cell Sorting (FACS).[3] It has also been used for separating visceral yolk sac layers.[2]

Biochem/physiol Actions

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.[4] Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.[7] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

sigma現(xiàn)貨代理Dispase II D-4693

我公司代理sigma公司試劑,大量現(xiàn)貨,和選購


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
CHINESE性旺盛老熟女| 亚洲码欧美码一区二区三区| 绝伦の上司に一晚人妻| 亚洲VA在线VA天堂VA无码| 午夜精品久久久久久99热蜜桃| 色翁荡熄又大又硬又粗又视频| 国产肉体XXXX裸体784大胆| 亚洲乱码中文字幕久久孕妇黑人| 白嫩少妇激情无码| 国产伦精品一区二区三区妓女| 野战肉高H男友和闺蜜| 中文字幕一区二区三区日韩精品| 国产精品扒开腿做爽爽爽| 诱人的大乳BD在线观看| 被几个领导玩弄一晚上| 哔哩哔哩免费观看在线视频| 内射人妻无码色AV麻豆| 被三个男人绑着躁我好爽视频| 亚洲AV无码精品色午夜果冻不卡| 斗罗大陆在线全集免费看| 日韩视频在线观看| 免费特级毛片| 女人18毛片A片久久18软件| 女人高潮的24种图片| 亚洲精品乱码久久久久久自慰| 宝贝∽好硬∽好爽一再来| 国产综合精品| 真人试看做受120秒3分钟| 狗狗太大了出不去| 麻豆亚洲AV熟女国产一区二| 纯爱无遮挡H肉动漫在线播放| 国产成人无码精品久久久免费| 女人和拘做受A级毛片视频| 小箩莉末发育娇小性色XXXX| 国产精品偷伦视频免费观看了| 日韩精品人妻系列无码专区免费| 少妇被猛烈挺进爽爽A片小说| 中文字幕乱码人妻二区三区| 日本少妇ASS浓精PICS| 男女无遮挡羞羞视频免费网站,| 亚洲欧美精品午睡沙发|