久久久久久亚洲精品,SM调教室虐男受调教H打开腿,欧洲-级毛片内射八十老太婆,护士趴下光屁股翘臀被打的作文

您好,歡迎進入上海力敏實業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁 >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 常規(guī)生化試劑 >> sigma現(xiàn)貨代理Dispase II D-4693

sigma現(xiàn)貨代理Dispase II D-4693

  • 更新時間:  2023-07-26
  • 產(chǎn)品型號:  
  • 簡單描述
  • sigma現(xiàn)貨代理Dispase II D-4693
    Related CategoriesApplication Index, Biochemicals and Reagents, Cell Biology, Dissociation, Enzymes, Inhibitors, and Substrates,
    More...
    activity ≥0.5 units/mg solid
    sol
詳細(xì)介紹

sigma現(xiàn)貨代理Dispase II D-4693

Description

Analysis Note

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.

Preparation Note

A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate and milk sugar

Application

The enzyme from Sigma has been used in developing a protocol for ex vivoculture of mouse embryonic mammary buds.[5] It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart.[6] It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.[1]
Dispase II has been used for Fluorescence-Activated Cell Sorting (FACS).[3] It has also been used for separating visceral yolk sac layers.[2]

Biochem/physiol Actions

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.[4] Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.[7] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

sigma現(xiàn)貨代理Dispase II D-4693

我公司代理sigma公司試劑,大量現(xiàn)貨,和選購


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
天天做天天摸天天爽天天爱| 国产精品久久久久一区二区三区| 挺进大幂幂的滋润花苞御女天下| 亚洲精品久久久口爆吞精| 丰满大肥婆肥奶大屁股| 中文字幕乱码人妻二区三区| 精品一线二线三线精华液| 大肉大捧一进一出好爽视频| 蜜臀AV色欲A片无码精品一区| 亚洲AV无码国产精品久久不卡| 嗯快点别停舒服好爽受不了了| 老头猛吸女大学奶头A片| CHINESE中年熟妇FREE| 老师洗澡时让我进去摸她那个| 国内精品国产成人国产三级| 国产日韩欧美一区二区三区| 男男开小嫩苞好深啊H窑子开| 免费A级毛片无码免费视频120软件| 女人与公狍交酡过程高清视频| 最强女婿完整版| 免费高清视频| 国产精品久久久久9999| 网禁拗女在稀缺视频网站| 国产免费AV| 久久96国产精品久久久| 成全视频在线观看在线播放高清| 日本55丰满熟妇厨房伦| 全黄H全肉1V1各种姿势| 被黑人猛烈进出到抽搐| 精品久久久无码21P发布| 熟妇人妻VA精品中文字幕| 色偷偷88888欧美精品久久久| 丰满女人又爽又紧又丰满| 国产小受呻吟AV视频在线观看| 狠狠人妻久久久久久综合蜜桃| 男女做爰猛烈叫床视频免费网站| 长篇乱肉合集乱500小说| 人妻被按摩师玩弄到潮喷| 少妇被躁爽到高潮无码人狍大战| 四川丰满少妇被弄到高潮| 高大丰满肥熟妇丰满大白屁股|